Türkçe       English

<< Geri Yazdır
Doi No: 10.5578/mb.24250

Karbapenemaz Üreten Enterobacteriaceae Suşlarının Saptanmasında
Karbapenem İnaktivasyon Yönteminin Değerlendirilmesi*

Evaluation of Carbapenem Inactivation Method for the Identification of
Carbapenemase-Producing Enterobacteriaceae Strains

Gülçin BAYRAMOĞLU1, Gülşen ULUÇAM1, Çiğdem GENÇOĞLU ÖZGÜR2


1 Karadeniz Teknik Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Trabzon.

1 Karadeniz Technical University Faculty of Medicine, Department of Medical Microbiology, Trabzon, Turkey.

2 Ahi Evren Göğüs Kalp ve Damar Cerrahisi Eğitim ve Araştırma Hastanesi, Mikrobiyoloji Laboratuvarı, Trabzon.

2 Ahi Evren Chest and Cardiovascular Surgery Education and Research Hospital, Microbiology Laboratory, Trabzon, Turkey.

* Bu çalışma, 31. ANKEM Kongresi (4-8 Mayıs 2016, Bodrum)'nde sunulmuştur.

ABSTRACT

The rapid and accurate identification of carbapenemases is of crucial importance in terms of infection control. Methods employed in the determination of carbapenemases should be constantly updated in the light of technical advances and newly emerging carbapenemase variants. The aim of this study was to evaluate the performance of the newly developed carbapenem inactivation method (CIM) for the identification of carbapenemases defined in the members of the family Enterobacteriaceae. Enterobacteriaceae isolates with resistance to at least one of the carbapenems (ertapenem, imipenem or meropenem) were included in the study. The study isolates were obtained from various clinical specimens between 2008-2014 and consisted of 56 Enterobacteriaceae strains (12 Escherichia coli, 32 Klebsiella spp., and 12 Enterobacter spp.) in which the presence of the 38 blaOXA-48, 8 blaVIM, 7 blaIMP, 1 blaNDM-1, 1 blaKPC-2 and 1 blaOXA-48+blaVIM genes had been previously determined using the polymerase chain reaction (PCR) and 78 in which no carbapenemase gene were detected. For the performance of the CIM, the test bacteria were suspended in sterile water and then a 10 μg meropenem disc was immersed in the suspension and incubated for 2 hours. This meropenem disc was then removed and subsequently placed on a Mueller-Hinton agar plate inoculated with E. coli ATCC 29522 and incubated at 35°C. The results were assessed after 6 hours and after overnight incubation. Development of an inhibition zone around the meropenem disk was interpreted as the absence of carbapenemase and the lack of an inhibition zone as the presence of carbapenemase. The results of the CIM were obtained after 8 hours. With the CIM, all isolates with previously determined carbapenemase genes were found to be positive and the isolates with no genes revealed to be negative. The sensitivity and specificity of CIM were estimated as 100%. The high sensitivity and specificity, ease of application and interpretation, rapid production of results, and no necessity for additional equipment or chemical substances, makes CIM a promising high throughput method to be used in routine microbiology laboratories. Since this method indicates only the presence of a carbapenemase in a clinical isolate, the type of the carbapenemase present should be further identified by the use of molecular techniques.

Keywords: Enterobacteriaceae; carbapenemase; carbapenem inactivation method

Geliş Tarihi (Received): 29.02.2016 • Kabul Ediliş Tarihi (Accepted): 03.06.2016

İletişim (Correspondence):

Doç. Dr. Gülçin Bayramoğlu,

Karadeniz Teknik Üniversitesi Tıp Fakültesi,

Tıbbi Mikrobiyoloji Anabilim Dalı,

61080, Trabzon, Türkiye.

Tel (Phone): +90 462 377 5653,

E-posta (E-mail): gulcinbay@hotmail.com

[ Tam metin ][ PDF ]
<< Geri Yazdır